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Antibody Affinity Maturation Service
Antigen binding affinity is one of the most critical properties of a therapeutic antibody. Therefore, methods used for antibody affinity maturation, including random mutagenesis, targeted mutagenesis, chain shuffling and in silico approaches, are widely applied.
We use Computer-based Modeling to generate a 3D structure of an antibody–antigen complex and predict mutation “hotspots”, generate a large number of antibody variants with specific mutations. This, combined with our State-of-the-Art Yeast Surface Display Platform, has resulted in significant improvements in antigen binding affinity.
Service Process
Fig 1. Antibody affinity maturation service process based on yeast surface display platform
Service Advantages
Fig 2. Service advantages of antibody affinity maturation
Computer-based Modeling
Antibody affinity maturation library design based on computational design.
Figure 3. Computer Modeling of Antibody–Antigen Interaction. Key residues at the interaction interface were predicted. Green: single-chain antibody; Blue: antigen N-terminal; Yellow: key residues in the antibody; Red: key residues in the antigen.
Affinity-improved mutation variants are obtained from yeast surface display platform.
Figure 4. Optimizing Antibody Binding Affinity. A yeast surface display library was constructed, followed by three rounds of FACS sorting. High-expression (anti-Kappa-PE) and high-binding (APC-Ag) clones were selected for functional validation.
In vitro Validation (Case Study)
Figure 5. Affinity Enhancement in Mutants vs. Wild Type. Mutants (M1, M2, M3) showed 20- to 200-fold affinity improvement over the wild type (WT).
Service provided
Tel：+86 4008677715
E-mail：service@nb-biolab.com
Address：SME Incubation Park, 319 Qingpi Avenue, Chengdu, China.
Working time：9:00-18:00