Home > services > Engineering Services

Engineering Services

NBbiolab is committed to providing professional services with the best quality to meet our clients’ requirement.


Antibody Affinity Maturation


Antigen binding affinity is one of the most critical properties of a therapeutic antibody. Therefore, methods used for antibody affinity maturation, including random mutagenesis, targeted mutagenesis, chain shuffling and in silico approaches, are widely applied.

NBbiolab uses Computer-Based Modeling to generate a 3D structure of an antibody–antigen complex and predict mutation“hotspots”generate a large number of antibody variants with specific mutations. This, combined with Our State-of-the-Art Yeast Surface Display Platform , has resulted in significant improvements of antigen binding affinity.


Antibody affinity maturation library design based on computational design.


1.png

Computer-Based Modeling predicts the key amino acid residues in the antibody–antigen interaction interface. The green band indicates the single chain antibody and the blue band indicates the N-terminal of the antigen. The yellow bar indicates key residues in the single-chain antibody, and the red band indicates key residues in the antigen. 


Affinity-improved mutation variants are obtained from yeast surface display platform.


2.png


In order to optimize the binding affinity of an antibody, a 3D structure of an antibody–antigen complex was analyzed. Antibody affinity maturation library (Fab) was constructed based on computer modeling. By performing 3 rounds of FACS sorting, affinity improved clones with high expression (anti-Kappa-PE) and binding activity (APC-Ag) were isolated for the following functional validation.


In vitro validation.

3.png

Mutations had achieved 20-200 fold improvement in affinity. 

1f06c09943dd4397941c0ec00af616c.jpg


Questions?

Contact us

Tel: +86 4008677715
+86 135 5108 6942
Address: SME Incubation Park, 319 Qingpi Avenue, Chengdu, China.
Email: service@nb-biolab.com
Our customer service representatives are available 24 hours a day, 7 days a week.

INFORMATION

Tel:+86 4008677715

E-mail:service@nb-biolab.com

Address:SME Incubation Park, 319 Qingpi Avenue, Chengdu, China.

Working time:9:00-18:00

Copyright © 2022 SICHUAN NB BIOLAB CO.,LTD For the record:蜀ICP备2022028448号-1Support:ANGOO