Revolutionize Your sdAbs Discovery with Our State-of-the-Art YSD Platform 

The unique properties of single domain antibodies (sdAbs) are outlined like relatively small in size, easy to handle, high affinity, high specificity, high thermostability, and good solubility. Furthermore, the modular nature of sdAbs allows them to be introduced into constructs that are simply not possible with conventional antibodies. With those advantages, sdAbs are employed in a rapidly growing range of applications in biotechnology and biomedicine.

NBbiolab is a well-recognized expert in the field of sdAbs development. Based on our advanced technology platforms and extensive experience, our scientists are devoted to provide professional and the best-suited solutions to advance our clients’projects.

A High-throughput Screening Method for Fast Identifification of sdAbs

Yeast surface display technology is a powerful tool for screening large libraries of proteins or peptides. In this technique, the protein or peptide of interest is fused to the surface of a yeast cell. By coupling this technique with flow cytometry and NGS, millions of sdAb clones can be sequenced in a single experiment, enabling the rapid identification of sdAbs against a target antigen.

 High-throughput platform for sdAbs discovery and optimization

The NBbiolab yeast surface display platform utilizes the "a-agglutinin" display system, where nanobodies are displayed at the N-terminus of Aga2p, fused with an HA tag at the C-terminus of the Aga2p subunit. The resulting fusion protein is secreted and anchored to the cell wall allowing nanobodies to be displayed on the cell surface thereby enabling their accessibility to soluble ligands.

 The mechanism of VHH yeast surface display

Differences Between Phage Dispaly and Yeast Surface Display

Antibody display libraries are traditionally analyzed by isolating a limited number of clones in combination

with Sanger sequencing, which may not detect all desirable clones due to dominance of certain clones and

random selecrion. By contrast, yeast surface display combined with next-generation sequencing (NGS)

allows for far-greater insights into library diversity enabling the identifification of high-affifinity and specifificity antibodies against a target antigen. 

Features of Yeast Surface Display Platform

1. Provides a direct connection between genotype and phenotype.  

2. Eukaryotic translation machinery offers expression in a similar fashion as mammalian cells.

3. No indication of amplification biases during antibody library screening.

4. Multivalent display with each cell displaying 10⁴ to 10⁵ copies of the VHH.

5. Accurate control over selection parameters during FACS screening.

6. Real-time kinetic observations during the selection process.

7. Selected binders further analyzed by NGS significantly increase the number of identified target-specific antibodies.

Advantages of NBBIOLAB sdAbs Service

1. Stable platform with extensive experience.

2. High-quality yeast display library, with a capacity of up to 10^8.

3. The library expression rates ranging from 65% to 80%, the highest industry standards.

4. Powerful combination of multi-color flow cytometry-based sorting and NGS sequencing.