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LOCATION: Home>Products>Nanoselector>HA tag Nanoselector Agarose
Nano-secondary antibody
Nanoselector
Smartcapture®
Recombinant VHH
Conventional Secondary Antibody
Recombinant Protein
Large Naïve VHH library

HA tag Nanoselector Agarose

Category: Nanoselector

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Product overview Documents References FAQ

Introduction

An HA-tag is derived from the human influenza hemagglutinin (HA) molecule corresponding to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors. The HA-tag does not appear to interfere with the bioactivity or the biodistribution of the recombinant HA-tagged protein.The HA tag's amino acid sequence is: YPYDVPDYA

HA tag Nanoselector Agarose  is a ready to use resin for immunoprecipitation/pulldown of HA tag fusion proteins


Specificity

The HA -Nanoselector recognizes the HA-tag sequence motif YPYDVPDYA at the N-terminus, C-terminus, or internal site of the fusion protein. 


Affinity

1.3nM


Binding capacity
High binding capacity,10 µL slurry bind about 20 µg of recombinant GFP-HA tag



Applications

Immunoprecipitation (IP) / Co-IP


Affinity purification

Mass spectrometry (MS)
On-bead enzyme assays
ChIP/ RIP analysis


Elution
- SDS sample buffer
- 0.2 M glycine pH 2.5

-40 µM YPYDVPDYA peptide

Instead of elution, we recommend on-bead assays like on-bead digestion for MS analysis.


Bead properties

Bead size: ~ 90 µm (cross-linked 4% agarose beads)

Storage buffer: 20% EtOH


Stability and Storage

Shipped at ambient temperature. Upon receipt store at +4°C. Stable for 1 year. Do not freeze


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Contact

If you want to sell VHH on our platform, please contact us !

Phone

13551086942

Email

service@nb-biolab.com

INFORMATION

E-mail:service@nb-biolab.com


Address:Wenjiang, Chengdu, Sichuan, China


Working time:9:00-18:00


CONTACT

Phone:400-166-9953

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