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LOCATION: Home>Products>Nanoselector>GST Nanoselector Agarose
Nano-secondary antibody
Nanoselector
Smartcapture®
Recombinant VHH
Conventional Secondary Antibody
Recombinant Protein
Large Naïve VHH library

GST Nanoselector Agarose

Category: Nanoselector

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Product overview Documents References FAQ

Introduction


Glutathione S-transferases (GSTs), previously known as ligandins, comprise a family of eukaryotic and prokaryotic phase II metabolic isozymes best known for their ability to catalyze the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates for the purpose of detoxification. GST can be added to a protein of interest to purify it from solution in a process known as a pull-down assay. This is accomplished by inserting the GST DNA coding sequence next to that which codes for the protein of interest. Thus, after transcription and translation, the GST protein and the protein of interest will be expressed together as a fusion protein.GST Nanoselector Agarose  is a ready to use resin for immunoprecipitation/pulldown of GST fusion proteins.The GST-Trap consists of a GST Nanobody /VHH coupled to agarose beads.


Specificity

Glutathione S-Transferase (GST) from Schistosoma japonicum


Affinity


0.26nM


Bead properties

Bead size: ~ 90 µm 

Storage buffer: 20% EtOH


Binding capacity
High binding capacity,10 µL slurry bind about 20 µg of recombinant GST


Elution
- SDS sample buffer
- 0.2 M glycine pH 2.5
Instead of elution, we recommend on-bead assays like on-bead digestion for MS analysis.


Stability and Storage

Shipped at ambient temperature. Upon receipt store at +4°C. Stable for 1 year. Do not freeze.


Benefits

  • Effective pull-down of GST fusion proteins, low background

  • Significantly higher sensitivity than glutathione cellulose resin and other affinity resins

  • NO heavy & light chain contamination

  • High binding affinity

  • Harsh washing conditions


Contact

If you want to sell VHH on our platform, please contact us !

Phone

13551086942

Email

service@nb-biolab.com

INFORMATION

E-mail:service@nb-biolab.com


Address:Wenjiang, Chengdu, Sichuan, China


Working time:9:00-18:00


CONTACT

Phone:400-166-9953

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