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LOCATION: Home>Products>Nanoselector>MBP Nanoselector Agarose
Nano-secondary antibody
Nanoselector
Smartcapture®
Recombinant VHH
Conventional Secondary Antibody
Recombinant Protein
Large Naïve VHH library

MBP Nanoselector Agarose

Category: Nanoselector

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Product overview Documents References FAQ

Introduction

MBP is used to increase the solubility of recombinant proteins expressed in E. coli. In these systems, the protein of interest is often expressed as a MBP-fusion protein, preventing aggregation of the protein of interest. The mechanism by which MBP increases solubility is not well understood. In addition, MBP can itself be used as an affinity tag for purification of recombinant proteins. The fusion protein binds to amylose columns while all other proteins flow through.MBP Nanoselector Agarose  is an affinity resin for immunoprecipitation of MBP fusion proteins. The MBP Nanoselector consists of an MBP Nanobody/ VHH coupled to agarose beads.


Specificity

Maltose binding protein (MBP), E. coli


Affinity


0.13nM


Bead properties

Bead size: ~ 90 µm 

Storage buffer: 20% EtOH


Binding capacity
High binding capacity,10 µL slurry bind about 20 µg of recombinant MBP


Elution
- SDS sample buffer
- 0.2 M glycine pH 2.5
Instead of elution, we recommend on-bead assays like on-bead digestion for MS analysis.


Stability and Storage

Shipped at ambient temperature. Upon receipt store at +4°C. Stable for 1 year. Do not freeze.


Benefits

  • Effective pull-down of MBP fusion proteins, low background

  • Significantly higher sensitivity than amylose resin and other affinity resins

  • NO heavy & light chain contamination

  • High binding affinity

  • Harsh washing conditions


Contact

If you want to sell VHH on our platform, please contact us !

Phone

13551086942

Email

service@nb-biolab.com

INFORMATION

E-mail:service@nb-biolab.com


Address:Wenjiang, Chengdu, Sichuan, China


Working time:9:00-18:00


CONTACT

Phone:400-166-9953

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