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LOCATION: Home>Products>Nanoselector>turboGFP Nanoselector Agarose
Nano-secondary antibody
Nanoselector
Smartcapture®
Recombinant VHH
Conventional Secondary Antibody
Recombinant Protein
Large Naïve VHH library

turboGFP Nanoselector Agarose

Category: Nanoselector

turboGFP

Product overview Documents References FAQ

Introduction

The dimeric green fluorescent protein TurboGFP is derived from the green fluorescent protein CopGFP of the copepod Pontellina plumata (Shagin et al., 2004). It possesses bright green fluorescence with excitation maximum at 482 nm and emission maximum at 502 nm. TurboGFP is a fast maturating protein: its fluorescent signal is visible earlier than other green fluorescent proteins. TurboGFP shares only about 20% sequence identity with jellyfish GFP variants. Therefore, most anti-GFP antibodies including the GFP-Nanobody used in GFP-Trap do not bind to TurboGFP. TurboGFP is mainly intended for applications where fast appearance of bright fluorescence is crucial. It is specially recommended for cell and organelle labeling and tracking the promoter activity. Destabilized TurboGFP variant allows accurate analysis of rapid and/or transient events in gene regulation.


Specificity

TurboGFP and CopGFP from the copepod Pontellina plumata.
The TurboGFP-Trap does not bind to jellyfish GFP and derivatives


Affinity

0.18nM


Bead properties

Bead size: ~ 90 µm 

Storage buffer: 20% EtOH


Binding capacity
High binding capacity,10 µL slurry bind about 25 µg of recombinant TurboGFP


Elution
- SDS sample buffer
- 0.2 M glycine pH 2.5
Instead of elution, we recommend on-bead assays like on-bead digestion for MS analysis.


Stability and Storage

Shipped at ambient temperature. Upon receipt store at +4°C. Stable for 1 year. Do not freeze.


Benefits

  • Effective pull-down of TurboGFP fusion proteins, low background

  • NO heavy & light chain contamination

  • High binding affinity

  • Harsh washing conditions

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Contact

If you want to sell VHH on our platform, please contact us !

Phone

13551086942

Email

service@nb-biolab.com

INFORMATION

E-mail:service@nb-biolab.com


Address:Wenjiang, Chengdu, Sichuan, China


Working time:9:00-18:00


CONTACT

Phone:400-166-9953

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