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LOCATION: Home>Products>Nanoselector>mNeogreen Nanoselector Agarose
Nano-secondary antibody
Nanoselector
Smartcapture®
Recombinant VHH
Conventional Secondary Antibody
Recombinant Protein
Large Naïve VHH library

mNeogreen Nanoselector Agarose

Category: Nanoselector

Product overview Documents References FAQ

Introduction

mNeonGreen is the brightest monomeric green or yellow fluorescent protein yet described to ourknowledge, performs exceptionally well as a fusion tag for traditional imaging as well as stochastic single molecule super-resolution imaging and is an excellent fluorescence resonance energy transfer(FRET) acceptor for the newest cyan fluorescent proteins. With the mNeogreen Nanoselector, specific for this non-GFP variant, yields of fusion proteins purification are high and free from heavy and light-chain contaminants, all in under 30 minutes. Save time, save effort and get cleaner results. The mNeogreen Nanoselector is the use of super-high affinity Alpaca antibody fragments called Nanobodies. mNeogreen Nanoselector may be used for immuno-precipitation, immuno-purification and immuno-pull down experiments with up to 10-fold better purity and yield than that of classic mouse monoclonal antibodies. Compatible with a variety of source materials, Nanoselector may be used with mammalian cells, tissues & organs, bacteria, yeast and even plants. These reagents allow your mNeonGreen-fusions to be perfect candidates for immune-precipitations, Co-IP, mass spectroscopy, and enzyme activity measurements.


Specificity

mNeogreen Nanoselector was tested on mNeonGreen 


Affinity

0.2nM


Bead properties

Bead size: ~ 90 µm 

Storage buffer: 20% EtOH


Binding capacity
High binding capacity,10 µL slurry bind about 20 µg of recombinant mNeogreen


Elution
- SDS sample buffer
- 0.2 M glycine pH 2.5
Instead of elution, we recommend on-bead assays like on-bead digestion for MS analysis.


Stability and Storage

Shipped at ambient temperature. Upon receipt store at +4°C. Stable for 1 year. Do not freeze.


Benefits

  • Clean immunoprecipitation of mNeonGreen-fusion proteins

  • No heavy & light chains in your downstream application

  • Strong binding even under harsh washing conditions

  • Short incubation time of about 30 minutes


Contact

If you want to sell VHH on our platform, please contact us !

Phone

13551086942

Email

service@nb-biolab.com

INFORMATION

E-mail:service@nb-biolab.com


Address:Wenjiang, Chengdu, Sichuan, China


Working time:9:00-18:00


CONTACT

Phone:400-166-9953

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