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LOCATION: Home>Products>Nanoselector>RFP Nanoselector Agarose
Nano-secondary antibody
Nanoselector
Smartcapture®
Recombinant VHH
Conventional Secondary Antibody
Recombinant Protein
Large Naïve VHH library

RFP Nanoselector Agarose

Category: Nanoselector

RFP

Product overview Documents References FAQ

Introduction

Red fluorescent proteins (RFPs) and variants thereof are widely used to study protein localization and dynamics. For biochemical analysis including mass spectrometry and enzyme activity measurements these RFP-fusion proteins and their interacting factors can be isolated fast and efficiently by immunoprecipitation using the RFP nanoselector Agarose. RFP nanoselector Agarose utilizes small recombinant alpaca antibody fragments covalently coupled to the surface of agarose beads.


Specificity
mCherry, mRFP, mRFPruby, mPlum, tagRFP, mKate2 and many more RFP derivatives


Bead properties

Bead size: ~ 90 µm 

Storage buffer: 20% EtOH


Binding capacity
High binding capacity,10 µL slurry bind about 25 µg of recombinant RFP


Elution
- SDS sample buffer
- 0.2 M glycine pH 2.5
Instead of elution, we recommend on-bead assays like on-bead digestion for MS analysis.

Stability and Storage

Shipped at ambient temperature. Upon receipt store at +4°C. Stable for 1 year. Do not freeze.


Benefits

  • Effective pulldown of RFP-fusion proteins for consistent results

  • No heavy & light antibody chains, short incubation (5-30 min)

  • Extraordinary binding, also under harsh conditions

  • Very high affinity (KD=0.15 nM) to bind even low abundant protein

RFP


Contact

If you want to sell VHH on our platform, please contact us !

Phone

13551086942

Email

service@nb-biolab.com

INFORMATION

E-mail:service@nb-biolab.com


Address:Wenjiang, Chengdu, Sichuan, China


Working time:9:00-18:00


CONTACT

Phone:400-166-9953

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