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LOCATION: Home>Products>Nanoselector>GFP Nanoselector Agarose
Nano-secondary antibody
Nanoselector
Smartcapture®
Recombinant VHH
Conventional Secondary Antibody
Recombinant Protein
Large Naïve VHH library

GFP Nanoselector Agarose

Category: Nanoselector

GFP

Product overview Documents References FAQ

Introduction

Green fluorescent proteins (GFPs) and variants thereof are widely used to study proteinlocalization and dynamics. For biochemical analysis including mass spectrometry and enzymeactivity measurements these GFP-fusion proteins and their interacting factors can be isolated fast and efficiently by immunoprecipitation using the GFP nanoselector Agarose .GFP nanoselector Agarose   utilizes small recombinant alpaca antibody fragments covalently coupled to the surface of Magnetic beads.


Specificity

GFP Smartcapture® beads was tested on eGFP, GFP, tagGFP, YFP, CFP, Venus, Citrine, AcGFP.


Bead properties

Bead size: ~ 90 µm 

Storage buffer: 20% EtOH


Binding capacity

High binding capacity,10 µL slurry bind about 25 µg of recombinant GFP


Elution

- SDS sample buffer
- 0.2 M glycine pH 2.5
Instead of elution, we recommend on-bead assays like on-bead digestion for MS analysis.


Stability and Storage

Shipped at ambient temperature. Upon receipt store at +4°C. Stable for 1 year. Do not freeze.


Benefits

  • Effective pulldown of GFP-fusion proteins for consistent results

  • No heavy & light antibody chains, short incubation (5-30 min)

  • Extraordinary binding, also under harsh conditions

  • Very high affinity (KD=1 pM) to bind even low abundant protein


GFP


Contact

If you want to sell VHH on our platform, please contact us !

Phone

13551086942

Email

service@nb-biolab.com

INFORMATION

E-mail:service@nb-biolab.com


Address:Wenjiang, Chengdu, Sichuan, China


Working time:9:00-18:00


CONTACT

Phone:400-166-9953

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